Talens

 Seq and RVDs

 Foward TALEN Sequence:

CCTCGGAGGACAGCTC

Spacer Sequence (restriction site):

AGTTTAGCGCTGGGG

Reverse TALEN sequence:

GTCCTACTCAGCCTTA

Forward TAL RVDs:

HD HD NG HD NN NN NI NN NN NI HD NI NN HD NG HD

Reverse TAL RVDs:

NN NG HD HD NG NI HD NG HD NI NN HD HD NG NG NI