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 Media Library Annotated Zebrafish Anatomical Ontology (ZFA) Expression Tags: digestive system, gut
Maternal mRFP:

Known Phenotypes:
Viability:


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Line Designation: GBT0433
Lab: Ekker lab - Mayo Clinic
GBT Plasmid: RP8.1
Tagged Gene: ddb1; damage-specific DNA binding protein 1 (from GenBank)
Tagged Gene Status: Confirmed
Alternative Gene Names: zgc:63840 (from GenBank)
Alternative Names: RP8 pr.2
Allele: mn0433Gt

Insertion Location:
  5' Genome:  
  3' Genome:  
RACE Tags:
  5'RACE:  
  3'RACE:  
Links:  ZFIN  Ensembl  Entrez Gene  

Additional Molecular Data
MO Information






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Expression Summary:
2 DPF4 DPF
D
S
V

Digital Notebook Entries:

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gbt0433 phenotype screen for week of 10/21/12

This week we had 4 inx pairs give with pr. 1 not making it through d1.

We observed a phenotype in all 3 pairs on d4 and 5.  However we did not see the same blood flow phenotype from a month earlier.  All three prs. exibited a small eye on d4 and d5.  On d7 these same pairs looked like they were dieing and had more pigment then the non phenotype fish.

Again these fish were 100% red and were not sortable.

pr. 2.  9/30 observed phenotype.

pr. 3.  6/30 observed phenotype.

pr. 4.  8/30 observed phenotype.

 

This line needs to be tested and confirmed

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GBT0433 phenotype screen for week of 9/16/12

This week we screened 4 inx pairs of gbt0433.  A phenotype was observed in all four pairs.  RFP was not sorted because inx pairs were 100% red (possibly maternal).  The phenotype was slow blood flow.  This phenotype was questionable as some did not agree or see the phenotype.

 

Pair. 1  7/30 contained phenotype

Pair. 2  1/31 contained phenotype

Pair. 3 8/30 contained phenotype

Pair. 4 16/27 contained phenotype

 

This phenotype needs to be reexamined with new inx. pairs and with other tools to measure blood flow.

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gbt 0433Phenotype screen week of 6/12/12

26/35 RFP+ and no observed phenotype.

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Last update: 01/27/2012