zfishbook
Connecting you to a world of zebrafish revertible mutants
 Media Library Annotated Zebrafish Anatomical Ontology (ZFA) Expression Tags: hair cell, lateral line system, muscle, musculature system, neuromast, peripheral nervous system
Maternal mRFP:

Known Phenotypes:
Viability:


Request This Line

Line Designation: GBT0936
Lab: Ekker lab - Mayo Clinic
GBT Plasmid: RP2.1
Tagged Gene: eya4
Tagged Gene Status: Candidate
Alternative Gene Names:
Alternative Names:
Allele: mn0936Gt

Insertion Location:
  5' Genome:
  3' Genome:
RACE Tags:
  5' RACE:
  3' RACE:

Links:  ZFIN Ensembl Entrez Gene

Additional Molecular Data
MO Information






 No Filter
Define Filter 
Expression Summary:
2 DPF4 DPF
D
S
V

Digital Notebook Entries:

View Printable Version

Confirmation of genotyping primers

Attached is a gel picture of the confirmatory PCRs (Accidentally, the directionality of these primers at first so the F1 in the picture is actually the reverse primer and the R1 is the fwd primer.)

However, the primer sequences listed are correct. Additionally, the primers didn't work well later. The reverse primer is good (bright bands in picture) but the fwd had faint bands so possibly the PCR needs to be optimized better or fwd needs to be redesigned.

My Account





Sign up as a New User
Lost your password?

Line Jump

Jump to line (# only):

Grant Support

Plasmids

All plasmids are available via Addgene

Have a Question or Suggestion?

Tell us about it...

Auto Translations

  • Arabic
  • Bulgarian
  • Catalan
  • Chinese Simplified
  • Chinese Traditional
  • Croatian
  • Czech
  • Danish
  • Dutch
  • Filipino
  • Finnish
  • French
  • German
  • Greek
  • Hebrew
  • Hindi
  • Indonesian
  • Italian
  • Japanese
  • Korean
  • Latvian
  • Lithuanian
  • Norwegian
  • Polish
  • Portugese
  • Romanian
  • Russian
  • Serbian
  • Slovak
  • Slovenian
  • Spanish
  • Swedish
  • Ukrainian
  • Vietnamese

What's New?

zfishbook updates
Last update: 01/27/2012